Awọn ohun elo Iwari Methylation DNA TAGMe (qPCR) fun Akàn Sẹ-ẹwẹ / Akàn Endometrial
Ọja ẸYA
Ti kii-afomo
Wulo pẹlu fẹlẹ cervical ati Pap smear awọn ayẹwo.
Rọrun
Imọ-ẹrọ wiwa methylation Me-qPCR atilẹba le pari ni igbesẹ kan laarin awọn wakati 3 laisi iyipada bisulfite.
Ni kutukutu
Awari ni ipele precancerous.
Adaṣiṣẹ
Ti o tẹle pẹlu sọfitiwia itupalẹ abajade ti adani, itumọ awọn abajade jẹ adaṣe ati kika taara.
Awọn oju iṣẹlẹ ohun elo
Tete waworan
Awọn eniyan ilera
Akàn Ewu Igbelewọn
Olugbe ti o ni eewu giga (dara fun papillomavirus eniyan ti o ni eewu giga (hrHPV) tabi rere fun cytology exfoliation cervical / rere fun papillomavirus eniyan ti o ni eewu giga (hrHPV) tabi rere fun cytology exfoliation cervical)
Abojuto ti nwaye
Prognostic olugbe
LILO TI PETAN
A lo ohun elo yii fun wiwa agbara in vitro ti hypermethylation ti jiini PCDHGB7 ni awọn apẹrẹ cervical.Fun akàn cervical, abajade rere tọkasi eewu ti o pọ si ti ipele 2 tabi ipele ti o ga julọ / diẹ sii ti ilọsiwaju cervical intraepithelial neoplasia (CIN2 +, pẹlu CIN2, CIN3, adenocarcinoma ni aaye, ati akàn cervical), eyiti o nilo colposcopy siwaju ati / tabi idanwo itan-akọọlẹ. .Ni ilodi si, awọn abajade idanwo odi fihan pe eewu ti CIN2 + kere, ṣugbọn ewu ko le yọkuro patapata.Ayẹwo ikẹhin yẹ ki o da lori colposcopy ati / tabi awọn abajade histopathological.Pẹlupẹlu, fun akàn endometrial, abajade rere kan tọkasi eewu ti o pọ si ti awọn egbo precancerous endometrial ati akàn, eyiti o nilo idanwo itan-akọọlẹ siwaju sii ti endometrium.Ni ilodi si, awọn abajade idanwo odi fihan pe eewu ti awọn ọgbẹ precancerous endometrial ati akàn jẹ kekere, ṣugbọn ewu ko le yọkuro patapata.Ayẹwo ikẹhin yẹ ki o da lori awọn abajade idanwo histopathological ti endometrium.
PCDHGB7 jẹ ọmọ ẹgbẹ ti idile protocadherin γ iṣupọ jiini.A ti rii Protocadherin lati ṣe ilana awọn ilana ti ibi-ara gẹgẹbi ilọsiwaju sẹẹli, ọmọ sẹẹli, apoptosis, ayabo, ijira ati autophagy ti awọn sẹẹli tumo nipasẹ ọpọlọpọ awọn ipa ọna ifihan, ati ipalọlọ jiini rẹ ti o ṣẹlẹ nipasẹ hypermethylation ti agbegbe olupolowo ni ibatan pẹkipẹki si iṣẹlẹ ati idagbasoke. ti ọpọlọpọ awọn aarun.O ti royin pe hypermethylation ti PCDHGB7 ni nkan ṣe pẹlu ọpọlọpọ awọn èèmọ, gẹgẹbi ti kii-Hodgkin lymphoma, akàn igbaya, akàn cervical, akàn endometrial ati akàn àpòòtọ.
Ilana iwari
Ohun elo yii ni reagent isediwon acid nucleic ati reagent wiwa PCR.Nucleic acid ni a fa jade nipasẹ ọna orisun orisun oofa.Ohun elo yii da lori ilana ti ọna PCR pipo fluorescence, ni lilo iṣesi PCR gidi-akoko gidi-methylation lati ṣe itupalẹ DNA awoṣe, ati nigbakanna ṣe awari awọn aaye CpG ti PCDHGB7 pupọ ati ami iṣakoso didara didara ti itọkasi awọn ajẹkù jiini G1 ati G2.Ipele methylation ti PCDHGB7 ninu ayẹwo, tabi iye Me, jẹ iṣiro ni ibamu si PCDHGB7 gene methylated DNA amplification Ct iye ati iye Ct ti itọkasi naa.PCDHGB7 jiini hypermethylation rere tabi ipo odi jẹ ipinnu ni ibamu si iye Me.
