Apo Iyọkuro Acid Nucleic (A01)
Ilana wiwa
Lẹhin itusilẹ DNA jinomiki nipa pipin awọn sẹẹli pẹlu ifipamọ lysis, ileke oofa le yan ni yiyan si DNA jiini ninu apẹẹrẹ.Nọmba kekere ti awọn idoti ti o gba nipasẹ ileke oofa le yọkuro nipasẹ ifipamọ fifọ.Ni TE, ileke oofa le tu silẹ DNA boundgenome, gbigba DNA jiini ti o ni agbara giga.Ọna yii rọrun ati iyara ati didara DNA ti a fa jade jẹ giga, eyiti o le pade ibeere fun wiwa DNA methylation.Nibayi, ohun elo isediwon ti o da lori ileke oofa le wa ni ibamu pẹlu isediwon acid nucleic laifọwọyi, ni ipade awọn iṣẹ-ṣiṣe isediwon acid nucleic ti o ga.
Awọn paati akọkọ ti reagent
Awọn eroja ti wa ni han ninu tabili 1:
Table 1 Reagent irinše ati ikojọpọ
| Orukọ paati | Awọn paati akọkọ | Ìtóbi (48) | Ìtóbi (200) |
| 1. Lysis ojutu | Guanidine hydrochloride, Tris | 11 milimita / igo | 44 milimita / igo |
| 2. Awọn ojutu mimọ A | NaCl, Tris | 11 milimita / igo | 44 milimita / igo |
| 3. Awọn ojutu mimọ B | NaCl, Tris | 13 milimita / igo | 26.5mL / igo * 2 |
| 4. Eluent | Tris, EDTA | 12 milimita / igo | 44 milimita / igo |
| 5. Protease K ojutu | Protease K | 1.1mL / nkan | 4.4mL / nkan |
| 6. Idaduro ileke oofa 1 | Awọn ilẹkẹ oofa | 1.1mL / nkan | 4.4mL / nkan |
| 7. Awọn ilana si yiyo nucleic acid reagents |
| 1 ẹda | 1 ẹda |
Awọn paati ti o nilo ninu isediwon acid nucleic, ṣugbọn ko si ninu ohun elo naa:
1. Reagent: ethanol anhydrous, isopropanol ati PBS;
2. Awọn ohun elo: 50ml centrifuge tube ati 1.5ml EP tube;
3. Ohun elo: Kettle iwẹ omi, pipettor, selifu oofa, centrifuge, 96-iho jin awo (laifọwọyi), ohun elo isediwon acid nucleic laifọwọyi (laifọwọyi).
Alaye ipilẹ
Awọn ibeere apẹẹrẹ
1. Agbelebu agbelebu laarin awọn ayẹwo yẹ ki o yee ni gbigba ayẹwo ati ibi ipamọ.
2.Iwadii naa yoo pari labẹ ibi ipamọ ọjọ 7 ti iwọn otutu ibaramu lẹhin gbigba ti awọn ayẹwo sẹẹli exfoliated cervical (ti kii ṣe ti o wa titi).Wiwa naa yoo pari labẹ ibi ipamọ ọjọ 30 ti iwọn otutu ibaramu lẹhin ikojọpọ apẹrẹ ito;Wiwa yoo pari ni akoko lẹhin ikojọpọ awọn ayẹwo sẹẹli ti o gbin.
Sipesifikesonu gbigbe pa:200 pcs / apoti, 48 pcs / apoti.
Awọn ipo ipamọ:2-30 ℃
Àkókò ìwúlò:12 osu
Ẹrọ to wulo:Tianlong NP968-C nucleic acid isediwon irinse, Tiangen TGuide S96 nucleic acid isediwon irinse, GENE DIAN EB-1000 nucleic acid isediwon irinse.
Ijẹrisi igbasilẹ ohun elo iṣoogun Bẹẹkọ./Ibeere imọ ẹrọ ọja No.: HJXB No.. 20210099.
Ọjọ ifọwọsi ati iyipada awọn ilana:
Ọjọ ifọwọsi: Oṣu kọkanla ọjọ 18, ọdun 2021
